テクニカルインフォメーション

WSE⁻5300 Printgraph CMOS I

A high-resolution monochrome CMOS camera equipped with motorized focus and motorized filter switching is suitable for fluorescence and visible light gel imaging. It supports imaging of various sizes and thicknesses of gels and membranes, including thick agarose gels and wide-sized acrylamide gels. It also comes with a guard, ideal for gel cutting.

Power supply / Imaging system

  • WSE-3100_PowerStation GhibliⅠ_How to use_ENG

    The World’s first Touch panel control!
    Simple operation with big touch color screen
    Operation system with a latex glove
    Broad output range
    Voltage 0~500V , Current 0~3000mA
    Available with High-speed mode electrophoresis and High-speed mode blotting.

  • u-PAGEL H_Amazing flexibility_ENG

    The ultimate gel "u-PAGEL H" with amazing physical strength and separability is now available!
    By using a new crosslinker, the new gel is stronger and harder to tear than conventional low-concentration gels.
    The gel is easy to handle during the operations of staining, bleaching and blotting.
    ※The gel is pulled to explain the strength, so please handle with care when operating.

  • How to make an SDS-PAGE gel_ENG

    This video explains how to make an SDS-PAGE gel using hand gel casting kit(AE-6401). 

  • WSP-3300_PeristaQuantumPump_Operation guide_ENG

    Digital new peristaltic pump Improved stability, reproducibility, chemical resistance, flow rate, operability, and external control functions.
    ■ Metal non-contact: Bio-Safe aseptic, contamination-free, sanitary and clean liquid feed
    ■ Accomplish the "High Flow Accuracy" by "Quantitative flow stability" and "High flow reproducibility"
    ■ Low pulsation: by "12-roller rotating roller system"
    ■ Includes "PeristaQ dedicated standard tube"
    ■ Easy to use by "Touch Panel Operation and Settings"
    ■ Productivity improved by external control system

  • WSP-3310_How to use PeristaQuantumPump_ENG
  • WSE-7010_Ezlabel FluoroNeo_How to use

    EzLabel FluoroNeo is a kit for SDS sample preparation and simultaneously for fluorescent labeling of protein and polypeptide. Hardly to detect any differences in protein band mobility after labeling reaction. The observed gel can proceed to western blotting as usual. After blotting, transferred proteins on membrane can be detected by excitation light..

  • Perista pump_Operation guide_ENG
    • Standard Perista Pump
    • Pumping fluids/solutions in chromatography
    • Pumping fermentation broth
    • Pumping chemicals in recirculation experiment
    • A wide range of application, from laboratory to plant, etc 
  • WSE-1190_Multi Mini-Slab Gel Cast_How to use_ENG

    Up to 4 pieces of Mini-Slab size (90 x 83 mm, thickness 1 mm) gels can be made simultaneously with this gel casting kit.

  • WSE-1150_PageRunAce_How to use_ENG

    WSE-1150 PageRunAce is a mini-slab size PAGE system with a built-in power supply.

  • WSL-2300_Phelios AL _Micro-volume measurement_ENG

    Only you need is 2μL 24 samples at a time In order to carry out experiments with high accuracy and reproducibility, it is necessary to measure the concentration of nucleic acids and proteins. However, the samples are limited and valuable and should be measured with minimal quantity and accuracy. The Phelios AL allows you to measure 24 samples at a time with a 2 μL sample volume using the “Nano Volume Plate” (optional). In preset mode, in addition to measuring the quantity, the spectrum for checking the purity of DNA / RNA can be automatically measured at the same time. The measured value is displayed as the average value measured three times. 

  • WSE-1500_DiscRun_How to use_ENG

    WSE-1500 DiscRun can do 1-D electrophoresis(IEF) for 2-D PAGE system.Two-dimensional electrophoresis can be done easily with the use of a version with a power source (electricity conditions entered) and “agarGEL” precast gel and a high level of reproducibility can be obtained 150-210 min. 

  • WSL-2300_Phelios AL_Measuring ABS_ENG

    WSL-2300 Phelios AL is a multimode microplate reader for measuring absorbance (ABS), luminescence (LUMI), and microvolume samples (NANO: 2µL). Phelios AL enables the use of a wide range of applications and is also superior in terms of performance and economy. 

  • WSE 1010_CompactPAGE Ace_How to use_ENG

    WSE-1010 cPAGE Ace is a compact-slab size PAGE system with a built-in power supply. The gel size(60mm x 60mm) and the apparatus body are very compact and easy-to-handle. 

  • WSE 1025_CompactPAGE Ace twin _How to use_ENG

    WSE-1025 CompactPAGE Ace Twin is a compact-slab size PAGE system with a built-in power supply. The gel size(60mm x 60mm) and the apparatus body are very compact and easy-to-handle.Up to 2 gels can be done electrophoresis.

  • WSE-1165_Mini-Slab_How to use_ENG

    This video explains how to use WSE-1165 Mini-Slab.

  • WSE-1710_Submerge-Mini_How to use_ENG

    Submarine electrophoresis system with built-in power supply! Submerge-Mini is available Hi-Speed(High voltage150V) electrophoresis, and has alarm timer function. 

  • AE-6100_Agarose EP kit(small type)_How to use_ENG

    Agarose EP Kit (small type)is an electrophoresis system for submerged, horizontal agarose gel.

  • AE-6111_Agarose EP kit(wide type)_How to use_ENG

    Agarose EP Kit(Wide type) is an electrophoresis system with buffer recirculation for submerged, horizontal agarose gel. 

  • AE-6500_Dual Mini Slab_How to use_ENG

    The AE 6500 was a best-selling model, but it has been redesigned to the AE-1165 and optimized for faster, higher resolution electrophoresis.

  • WSC-2900_Rotator atto_How to use_ENG

    Tabletop small volume rotary mixer  "WSC-2900 Rotator atto" .
    Mixing of 1.5mL, 15mL and 50mL tubes are available.

  • AE-1460_EzBlot_How to use_ENG

    EzBlot is a transfer buffer comprised of three reagent for semi-dry blotting of protein sample. 

  • WSC-2800_MyMiniVortex_How to use_ENG

    Compact vortex mixer "WSC-2800 MyMiniVortex".
    It's compact size that fits in the palm of your hand and mixing of 1.5mL~50mL tubes are available! 

  • WSE-4057_QBlot kit M_How to use_ENG

    QBlot kit series are transfer pack. Needless preparation! Convenient and easy to use.

  • Western blotting method_ENG

    This video explains how to operate Western blotting using ATTO products. 

  • WSC-2615_MyMiniBLOCK C&H_How to use_ENG

    A personal-sized compact block incubator. Temperature setting from 0 to 100 ℃, available for 0.2mL tube to 50mL tube. Convenient and easy to use. 

  • WSC-2700_MyMiniSpin_How to use_ENG

    This video explains how to use MyMiniSpin (WSC-2700)

  • WSE-7160_EzStainAQua MEM_How to use_ENG

    Total protein stain kit for transfer membrane. visualization of protein in transfer membrane. check transfer unevenness before antibody reaction. 

  • u-PAGEL H_How to handle the gel for western blotting_ENG

    The ultimate gel "u-PAGEL H" with amazing physical strength and separability is now available! The low concentration gels are softer than high concentration gels,so it's difficult for handling. How to handle the gel for western blotting! 

  • WSL-1850_Wound healing captured by CytoWatcher II
  • WSF-2000 MH_Fermograph_3_ENG

    Fermentograph is a measuring device for the amount of gas produced through microbial fermentation and similar processes.
    The Fermentograph III is a successor model adopted by the Japan Yeast Industry Association for the "Bread Yeast Testing Method".
    It is capable of automatically measuring the gas production of up to 20 samples simultaneously and in parallel.

  • u-PAGEL H_Amazing flexibility_ENG

    The ultimate gel "u-PAGEL H" with amazing physical strength and separability is now available!
    By using a new crosslinker, the new gel is stronger and harder to tear than conventional low-concentration gels. 
    The gel is easy to handle during the operations of staining,  bleaching and blotting.
    ※The gel is pulled to explain the strength, so please handle with care when operating.

  • WSL-1850_Fluorescent Imaging of GFP expressed NIH3T3 cells
  • WSL-1850_CytoWatcher Ⅱ_How to use_ENG

    CytoWatcherⅡ/CytoWatcherⅡ FL is a digital microscope that allows for the long-term imaging of live cells and tissues without causing damage. Designed to be space-efficient and energy-saving, it fits compactly inside a CO2 incubator, minimizing the impact on environmental temperature. It supports not only bright-field imaging but also fluorescence imaging, and its outstanding color reproduction is suitable for observing tissue sections and living tissues.

  • WSL-1800_CytoWatcher_Time-lapse cell imaging of GFP cells (FL/BF merged)

    Fluorescence and bright field time-lapse images of NIH 3T3 cells (a 35 mm glass bottom dish) for 48 h after transfection of EGFP expression vector, using a fluorescent imaging model of CytoWatcher. ATTO CytoWatcher.

  • AE-2550_Kronos Dio_Operation guide_ENG
  • WSL-1800_CytoWatcher_Time-lapse cell imaging of GFP cells

    Fluorescence time-lapse images of NIH 3T3 cells (a 35 mm glass bottom dish) for 48 h after transfection of EGFP expression vector, using a fluorescent imaging model of CytoWatcher. ATTO CytoWatcher.

  • WSL-1800_CytoWatcher_Time-lapse cell imaging of IVF bovine embryos

    Time-lapse live cell imaging system, CytoWatcher (ATTO) Time-lapse monitoring bovine embryos 6 hours after in vitro fertilization for 9 days (0.5 h-intervals) in an incubator By courtesy of Kei Imai, Department of Sustainable Agriculture, Rakuno Gakuen University, Japan ATTO CytoWatcher.

  • AB-3000_Cellgraph_Monitoring of wound healing assay with luminescence in living cell_ENG

    The result of the wound healing assay observed with Cellgraph are shown in this movie. NIH3T3 cells stably expressing luciferase were cultured until fully confluent. Wounds were created by scraping monolayer cells with a sterile pipette tip. Then wound closure was monitored by Cellgraph.

  • AB-3000_Cellgraph_Quantitative Data analysis of luminescent intensities in living cells_ENG

    Cellgraph Viewer is an image analysis software. It is very simple and easy to use, and has multiple function such as luminescent intensity measurement, making movie and montage... etc.. The ROI mode in analysis tools provides the function to measure bioluminescent intensities individually in any given region of interest. The result of analyzing bioluminescence intensity data can be exported as CSV format files.

  • wsl-1800-cytowatcher-time-lapse-cell-imaging-of-wound-healing-assay

    Time-lapse live cell imaging system, CytoWatcher (ATTO) Wound healing assay with fibroblast NIH3T3 cells for 24 h (30 minutes-intervals) in a CO2 incubator ATTO CytoWatcher.

  • AB-3000_Cellgraph_Real time monitoring assay of luminescence in living cells_ENG

    Bioluminescent images are acquired from NIH3T3 cells expressing SV40 promoter fused luciferase with cellgraph. The merge images of bright field and bioluminescence shown as pseudo color.

  • AB-3000_Cellgraph_Visualization of ATP oscillation with luciferase in living cell_ENG

    Cellular condensation in embryonic limbs that occurs in the early stage of chondrogenesis is considered to play a critical role in the secretion of adhesion molecules and extracellular matrixes. The movie shows the visualization of ATP oscillation after the induction of chondrogenesis in ATCD5 cells transfected with an ATP-dependent Phyxothrix hirtus luciferase gene. As demonstrated in this study, the Cellgraph system is an effective.

  • Time-lapse cell imaging, NIH3T3 cells by CytoWatcher

    Time-lapse live cell imaging system, CytoWatcher (ATTO)
    Growth of fibroblast NIH3T3 cells for 72 h (30 minutes-intervals) in a CO2 incubator

  • AB-3000_Cellgraph_Visualization of intracellular protein trafficking in living cells_ENG

    Visualization of nucleocytoplasmic shuttling of importin α by the Cellgraph system. In this study, importin α gene fused with luciferase was expressed in NIH3T3 cells. The time-lapse images were acquired using three minutes exposure time at intervals of four minutes with a 40x objective lens without binning. The luminescence signal was initially detected in the cytosol, then in the nucleus. After that, the luminescence signal in the nucleus gradually increased. As shown above, the Cellgraph system is an ideal tool for observing biological events such as trafficking of proteins that occur over a prolonged period of time.
    Reference: Y. Nakajima, PLoS One, 5(4), e10011 (2010) [PubMed]

  • AB-3000_Cellgraph_Bioluminescence imaging in brain tissue sample_ENG

    Using the Cellgraph system, a brain tissue slice from a transgenic mouse that express luciferase under control of the clock gene promoter were analyzed. The Brain was removed and sectioned into 100 µm thick slices using a Microslicer, each of which was then placed in a culture insert. The time-lapse images of an SCN section acquired over a period of five days using the Cellgraph system. Using the grid measurement function of “Cellgraph Viewer”, the bioluminescence intensity in each area was analyzed and quantified.

  • AB-3000_Cellgraph_AB-2550_KronosDio_Apoptosis analysis_ENG

    Cells were transfected with luciferase reporter gene fused to nuclear targeting signal sequence. Cells were stimulated with STS (1μM) and observed by Cellgraph. Cellgraph system enable us to visualize apoptotic phenomena such as nuclear fragmentation and membrane rupture in apoptotic cells by STS stimulation.

  • AB-3000_Cellgraph_Bioluminescent imaging of activated GnRH neurons by kisspeptin stimulation

    Ultradian rhythms of gonadotropin-releasing hormone (GnRH) gene transcription in single GnRH neurons were monitored using cultured hypothalamic slice prepared from transgenic mouse expressing a GnRH promoter-driven destabilized liciferase reporter. It was demonstrated that GnRH gene transcription was synchronously activated after kisspeptin was added to the media.
    Reference: HK. Choe, Proc. Natl. Acad. Sci. U.S.A., 110(14), 5677-5682 (2013) [PubMed]