Agarose Gel Electrophoresis (Submarine electrophoresis)
What's Agarose Gel Electrophoresis (Submarine Electrophoresis)?
Horizontal agarose gel electrophoresis for nucleic acid (DNA/RNA) sample is operated by the method to immerse gel in buffer. This method is also called submarine electrophoresis compared to a submarine in the sea. We, ATTO, provide the system of method called "Submerge Series".
Nucleic acid (DNA/RNA) dissolving in buffer etc. is charged in negative by phosphate residue. If the solution (DNA sample) is added to agarose gel and electrophoresed in buffer, it migrates towards anode (+). At this time, long DNA moves slowly through network structure on the contrary short DNA moves rapidly due to molecular sieve effect of gel. So, nucleic acid shows each mobility depending on the size of nucleic acid. Electrophoresis pattern of nucleic acid is provided by dyeing gel after electrophoresis with fluorescent dye (Ethidium bromide) and detecting it. You can determine the size (Molecular weight marker is necessary) and get the information of genotype for individual identification from this pattern.
Example of Use ： Genotyping
Submerge series is suitable for electrophoresis for analyzing genetic polymorphism because gel size is large and migration distance can be made longer. Also many number of samples are operated at once so it is appropriate to electrophoresis for confirming PCR products. The general method for genotyping is following; DNA extraction from sample-PCR (Polymerase Chain Reaction)-Agarose gel electrophoresis-Gel imaging/analyzing.
ATTO Submarine Type Electrophoresis System
Agarose EP Kit
Agarose EP Kit
|Gel casting style||Tray style||Tape style||Dam style|
S:9/5well x2, L:22/12well x2
(Double edged comb)(3/5.8mm width)
(Double edged comb)(3/6mm width)
|-||-||Available; Nozzle for buffer circulation is attached|
|Power supply unit||Built-in||-||-|
|Weight||0.45kg(Except AC adaptor)||0.5kg||1.5kg|
How to Select Electrophoresis Chamber
Please select electrophoresis system depending on sample. For example, in the case of the experiment with many number of samples (well), we recommend the system of which width (W) is broad. In the case of experiment with many number of bands, the system of which length (L) is long is suitable.
How to Select Power Supply
Power supply is required when Submerge series is used. Please note that the larger the system (gel) is, the larger current capacity it requires.
|AE-8135||myPower II 300||○||○|
|AE-8155||myPower II 500||○||△|