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Reagents TMB Substrate for HRP Detection
AE-1490 EzWestBlue

Peroxidase substrate (TMB)
EzWestBlue is a TMB substrate for horseradish peroxidase (HRP) used for colorimetric detection of immunobloting.

AE-1490 EzWestBlue

AE-1490 EzWestBlue

Comparison of detection efficiency with DAB

EzWestBlue is higher sensitivity to detect signals than DAB

Purpose and Application
  • EzWestBlue is a TMB substrate for horseradish peroxidase (HRP) used for colorimetric detection of immunobloting.
    Note: not applicable to ELISA
Features
  • Substrate for horseradish peroxidase (HRP)
  • Ready to use
  • Highly sensitive, reducing the used amount of valuable antibodies
  • Visualized in bright and clear blue
  • Safer than diaminobenzidine (DAB)
  • Absence of enzyme inhibition due to the absence of organic solvent
Specifications
Product number AE-1490
Product Name EzWestBlue
Major components TMB solution (not containing organic solvents)
 (TMB 3,3',5,5' tetramethylbenzidine)
Method of use Appropriate amount is applied to the membrane in the tray (approximately 0.1 mL/1 cm2 membrane)
 Water-washed after visualization
Applicable amount Serve for 2000 cm2(approximately 25 sheets of mini-gel-size membrane)
Storage period At 4°C for 6 months
Package 200 mL/bottle 

Documents

Product Brochure

(0.85 MB)

Instruction Manual

(0.16 MB)

Download

You can download various documents (PDF)

  • ATTO Product Catalog

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