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Reagents Subcellular Fractionation/Extraction Kit
WSE-7420 EzRIPA Lysis kit

RIPA lysis buffer
RIPA lysis buffer for protein extraction from mammalian cells or tissue samples

RIPA lysis buffer

WSE-7420 EzRIPA Lysis kit

Purpose and Application
  • Whole cell extract preparation for the following applications
     Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Enzyme activity/ etc.
Features
  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
  • Standard set includes Protease inhibitor and Phosphatase inhibitor.
  • Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.
Specifications
Product Number WSE-7420
Product Name EzRIPA Lysis kit
Applicable amount 100 samples, up to 2 x 107 cell/sample
Storage 1 year
Package ●EzRIPA Lysis buffer: 100mL
 20mM HEPES (pH7.5)
 1% IGEPAL CA-630
 0.1% SDS
 0.5% Deoxycholic acid
 150mM Sodium Chloride
●Protease Inhibitor (100×):1mL
 Aprotinin, PepstatinA, Leupeptin
●Phosphatase Inhibitor (100×):1mL
 Sodium fluoride, Sodium orthovanadate(V),β-Sodium glycerophosphate
IGEPAL CA-630 is the same as NonidetP-40, and a trade mark registration of Rhone-Poulenc AG Co.

Documents

Product Brochure

(1.59 MB)

Instruction Manual

(0.21 MB)

Download

You can download various documents (PDF)

  • ATTO Product Catalog

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