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Reagents Stripping Reagent
WSE-7240 EzReprobe

Efficient Stripping Buffer 
EzReprobe is a high performance stripping reagent for western blotting

Efficient Stripping Buffer

WSE-7240 EzReprobe

Purpose and Application
  • EzReprobe is a stripping reagent to remove the 1st and 2nd antibody from the membrane after western blotting reaction
  • Repeatable stripping and reprobing reaction
Features
  • Less damage to the antigen on the blot
  • Repeatable stripping and reprobing reaction
  • Ready to use
  • Soak the used blot in EzReprobe and incubate for 10 min at room temperature
  • Economical; good cost performance
  •  Toxic substances FREE ( like β-mercaptoethanol)
  • Efficient stripping performance
    Rabbit monochlonal antibody which is generally shown high titer can be removed.   
Specifications
Product Number WSE-7240
Product Name EzReprobe 
Major components Buffer, Surfactant 
Method of use Working solution:Mix 100mL of EzReprobe with 0.6g of enhancer
Storage period At room temperature for 1 year
Package EzReprobe reagent: 500 mL
Enhancer (Powder): 3g

Product Summary

Application

EzReprobe is suitable for the following situations.

  • Amount of sample is limited
  • Quantitative analysis is performed with plural antibodies
  • Western blotting results in failure (ex.,blocking reaction was too strong, Dilution rate of antibody was imappropriate) 
Principle
reprobe 3

1. Immense membrane after emission detection in EzReprobe solution and shake it  to incubate for 15 min at room temperature.

    *Incubation time depends on antibody titer.

    *In the case of antibody having high antibody titer or difficult to come off, incubation time should be extended.

2. After cleaning with wash buffer, the step after blocking is performed for antigen-antibody reaction.

Example data

Even rabbit monoclonal antibody having high antibody titer can be stripped with EzReprobe. 

Also, denaturation of antigen is minimized.

reprobe4
  • Sub band of human GAPDH is not detected at 4th step with EzReprobe.
  • EzReprobe has a large amount of expression of antigen such as GAPDH, and stripping can be performed beautifully under the condition which antibody titer is high if reaction time of stripping is extended.

*Red arrow head: Shows the band of target antigen of each step.

*Grey arrow head: Shows the band result from antibody cannot be peeled off by stripping operation.

*Non-Treated: Shake it to incubate at room temperature with wash buffer (TBST)

*Handmade:  Shake it to incubate for 30 min at 50 ℃ (refer to Kaufmann et al., 1987)

*The other 3 reagents and EzReprobe: Shake it to incubate for 10 min at room temperature

Documents

Product Brochure

(0.93 MB)

Instruction Manual

(420.07 kB)

Download

You can download various documents (PDF)

  • ATTO Product Catalog

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