Bioluminescence measurement system for living cell and tissues
Kronos Dio is performing to detect high sensitivity for dim and weak luminescence consecutively in culture samples such as cells and tissues in 35mm culture dishes for a long time. 24・96 well micro plates also applicable.
*GPC laboratory CO. LTD. provide useful cell line carrying luciferase as a reporter gene for assessing circadian rhythm by Bmal1 gene expression.
Purpose and Application
- Sample stage is in the incubation chamber in which temperature and CO2 gas concentration is controlled and humidity is kept >90%RH.
- 24・96 wells micro plate applicable.
- Long-time detection available *1hour～30days
- 2 plates are measured by 2 PMTs (one PMT to one plate)
- Plates are not moved, PMTs are moved to each well. > No shaking cells, so less damage to delicate cells and applicable to floating cells such as hematocytes.
- PMTs are cooled to 10℃ > The dark noise is lower.
- Built in filters for Multi-color luciferase assay
|Model||WSL-1565 Kronos HT|
|Detector||PhotoMultiplier Tube (10℃ cooling) x 2units|
|Measurment||Photon counting method|
|Measurement time||1-60s/well, for 1h-30days|
24 well clear bottom plate x 2 (Standard）
96 well clear bottom plate x 2 (Option）
35mm dish x 12 (Option）
|Detection plate||2 PMTs (one PMT to one plate)|
48 samples (24 well plate ×2）
192 samples (96 well plate ×2）Option
12 samples (35mm dish ×12）Option
|Incubation temperature control||
Room temperature +5℃ to 45℃
at room temperature 20 - 28℃
|CO2 gas control||Control to 5% by CO2 gas mixer|
Setting of water receiver in incubator and humidified 5％ CO2 gas flowing intermittently by humidifying out
Maintaining > 90％RH
|Multi-color assay||Measurement with aoutomatically selected optical filter up to 2 colors|
OS：Windows10/8.1/7 , 64/32bit, Memory：4GB,
HDD：more than 20GB
|PC interface||USB2.0 x 1 port|
|Demention・Weight||650(W)×520(D)×340(H)mm ・ 40kg|
|Input||AC100～240V 50/60Hz 400W（Max）|
|Model||CO2 gas mixer unit for Kronos HT|
|CO2 Control||Keep 5% of CO2|
Insert CO2 gas into Kronos HT incubator chamber
through humidification unit
CO2 gas mixer unit：160(W)×300(D)×200(H)mm 4.8kg
Humidification unit：170(W)×140(D)×115(H)mm 1.6kg
CO2 gas mixer：AC100~240V 50/60Hz 100W Max
Humidification unit：AC100~240V 50/60Hz 24W Max