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Time-lapse live cell imaging system

Live Cell Research Time-lapse live cell imaging system
WSL-1800CytoWatcher

WSL-1800 CytoWatcher is a compact digital microscope.

No stress to take space in laboratory and moisture resistant for setting in a COincubator for time-lapse live cell imaging.

WSL-1800 CytoWatcher
Purpose and Application

> For bright field cell imaging with low magnification.

> Compact size, no stress to take space.

> Usable in CO2 incubator, for time-lapse imaging in culture condition.

> Equipped with 5M pixels color CMOS camera.

> Multiple samples can be taken by connecting multiple units.

> A model for fluorescent imaging has appeared.

Product Summary

WSL-1800 CytoWatcher is a digital microscope equipped with a 5 megapixel color CMOS camera.

By simply connecting to a Windows PC with a USB cable, you can capture and save microscopic images with a simple operation.

Since CytoWatcher is moisture-proof, it can be placed in a CO2 incubator to take time-lapse images automatically while culturing cells.

Features
Usable in CO2 incubator

Since CytoWatcher body is moisture-proof, it can take time-lapsed images of live cells with cultured in a high humidity CO2 incubator. There is no need to use an expensive microscope stage culture system.

CytoWatcher_インキュベーター+タブレット壁掛け

 

CytoWatcher_インキュベータに設置

 

Compact microscope

As CytoWatcher is compact, it can be used in a limited space such as a labolatory bench or a safety cabinet. As it is easy to carry, you can take it to your work space and use it.

CytoWatcher_クリーンベンチ内

 

Just connect to PC with USB

CytoWatcher is used by connecting it to a Windows PC (option) with a USB 3.0 cable (power is supplied from the PC via USB). If you start the control software ImageSaverT (included as standard), you can see live cell images immediately. You can also save images and take time-lapse images easily.

CytoWatcher+タブレット

 

Multiple units can be controlled by one PC

Multiple CytoWatchers can be connected to one PC and used (up to 4 units) for simultaneous time-lapse imaging of multiple samples in parallel.

CytoWatcher_2台接続

 

Fluorescent imaging

The fluorescent imaging model is equipped with a blue LED light source and an excitation / emission filter, and supports green emitted fluorescent imaging such as GFP. It is possible to set it up in a CO2 incubator and combine it with bright field and fluorescent time-lapse imaging.

CytoWatcher_FL

 

 

 

 
 
 
 

 

 

 

 

 

 

 

 

 

 

 

5M Pixels color digital camera

CytoWatcher is applied 4x object lens with 5M pixels color CMOS camera.

Field of view: 1.425 mm × 1.070 mm

1_4

 4x image

 

1_2

 8x image (digital zoom)

 

1_1

 16x image (digital zoom)

Specifications
Model Bright field imaging Fluorescent / bright field imaging
Name WSL-1800 CytoWatcher WSL-1800-B CytoWatcher FL
Camera 5M pixels color CMOS camera
Resulution 2592 x 1944 pixels
Magnification 4 x
Field of view 1.425 mm x 1.070 mm
Focusing Manual adjustment
Light source     White LED (trans-illumination) White LED (trans-illumination)
Side illuminating epi-blue LED (465 nm)
Optical filter - Excitation: 480 nm shortpass filter
Emission: 525 / 45 nm bandpass filter
Moisture
resistant
Usable in humidity 95%RH (usable in a CO2 incubator)
Control software ImageSaverT for Windows (included as standard)
Capture mode: Live / Time-lapse
Save image file: 8 bit TIFF / BMP / JPEG, Color / Monochrome
PC connection USB3.0 x 1 USB3.0 x 1, USB2.0 (or 3.0) x 1
OS Windows 10 / 8.1 / 7 (64 / 32 bit)
Size, Weight 130 (W) x 180 (D) x 190 (H) mm, 2.5 kg Main unit: 130 (W) x 180 (D) x 190 (H) mm, 2.7 kg
Power     USB (bus powered)
Brochure

WSL-1800 CytoWatcher brochure

 

Applications

 

[1] Growth of NIH3T3 cells

Mouse fibroblast NIH3T3 cells on a 10 cm dish, Imaging for 72 h (30 minutes-intervals)

 

[2] Wound healing assay

Imaging for 24 h (30 minutes-intervals) after scratching the cultured layer of 100% confluent NIH3T3 cells on a 35 mm dish

 

[3] Differentiation into Adipocyte-like cells

Swiss 3T3 L-1 cells imaging for 7days (1 h-intervals) after replacing to the differentiation inducing medium (DMEM, 10 % FBS, 250 nM Dexamethazone, 0.5 mM Isobutylmethylxanthin, 10 ug/mL Insulin)

CytoWatcher_脂肪滴染色画像

Taken after staining lipid droplets with Oil Red O

 

[4] GFP fluorescent cell imaging

Fluorescence and bright fieeld images (using a fluorescent imaging model of CytoWatcher) of NIH 3T3 cells (a 35 mm glass bottom dish) into which EGFP expression vector has been transfected.

CytoWatcher_蛍光+明視野撮影画像

 

[5] Strained tissue section

Masson trichrome stained section preparation of basal ganglia (mammal)

CytoWatcher_染色切片画像

Download

You can download various documents (PDF)

  • ATTO Product Catalog

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