Time-lapse live cell imaging system
Live Cell Research
Time-lapse live cell imaging system
WSL-1800 CytoWatcher is a compact digital microscope.
No stress to take space in laboratory and moisture resistant for setting in a CO2 incubator for time-lapse live cell imaging.
Purpose and Application
> For bright field cell imaging with low magnification.
> Compact size, no stress to take space.
> Usable in CO2 incubator, for time-lapse imaging in culture condition.
> Equipped with 5M pixels color CMOS camera.
> Multiple samples can be taken by connecting multiple units.
> A model for fluorescent imaging has appeared.
WSL-1800 CytoWatcher is a digital microscope equipped with a 5 megapixel color CMOS camera.
By simply connecting to a Windows PC with a USB cable, you can capture and save microscopic images with a simple operation.
Since CytoWatcher is moisture-proof, it can be placed in a CO2 incubator to take time-lapse images automatically while culturing cells.
Usable in CO2 incubator
Since CytoWatcher body is moisture-proof, it can take time-lapsed images of live cells with cultured in a high humidity CO2 incubator. There is no need to use an expensive microscope stage culture system.
As CytoWatcher is compact, it can be used in a limited space such as a labolatory bench or a safety cabinet. As it is easy to carry, you can take it to your work space and use it.
Just connect to PC with USB
CytoWatcher is used by connecting it to a Windows PC (option) with a USB 3.0 cable (power is supplied from the PC via USB). If you start the control software ImageSaverT (included as standard), you can see live cell images immediately. You can also save images and take time-lapse images easily.
Multiple units can be controlled by one PC
Multiple CytoWatchers can be connected to one PC and used (up to 4 units) for simultaneous time-lapse imaging of multiple samples in parallel.
The fluorescent imaging model is equipped with a blue LED light source and an excitation / emission filter, and supports green emitted fluorescent imaging such as GFP. It is possible to set it up in a CO2 incubator and combine it with bright field and fluorescent time-lapse imaging.
5M Pixels color digital camera
CytoWatcher is applied 4x object lens with 5M pixels color CMOS camera.
Field of view: 1.425 mm × 1.070 mm
8x image (digital zoom)
16x image (digital zoom)
|Model||Bright field imaging||Fluorescent / bright field imaging|
|Name||WSL-1800 CytoWatcher||WSL-1800-B CytoWatcher FL|
|Camera||5M pixels color CMOS camera|
|Resulution||2592 x 1944 pixels|
|Field of view||1.425 mm x 1.070 mm|
|Light source||White LED (trans-illumination)||
White LED (trans-illumination)
Side illuminating epi-blue LED (465 nm)
Excitation: 480 nm shortpass filter
Emission: 525 / 45 nm bandpass filter
|Usable in humidity 95%RH (usable in a CO2 incubator)|
ImageSaverT for Windows (included as standard)
Capture mode: Live / Time-lapse
Save image file: 8 bit TIFF / BMP / JPEG, Color / Monochrome
|PC connection||USB3.0 x 1||USB3.0 x 1, USB2.0 (or 3.0) x 1|
|OS||Windows 10 / 8.1 / 7 (64 / 32 bit)|
|Size, Weight||130 (W) x 180 (D) x 190 (H) mm, 2.5 kg||Main unit: 130 (W) x 180 (D) x 190 (H) mm, 2.7 kg|
|Power||USB (bus powered)|
WSL-1800 CytoWatcher brochure
 Growth of NIH3T3 cells
Mouse fibroblast NIH3T3 cells on a 10 cm dish, Imaging for 72 h (30 minutes-intervals)
 Wound healing assay
Imaging for 24 h (30 minutes-intervals) after scratching the cultured layer of 100% confluent NIH3T3 cells on a 35 mm dish
 Differentiation into Adipocyte-like cells
Swiss 3T3 L-1 cells imaging for 7days (1 h-intervals) after replacing to the differentiation inducing medium (DMEM, 10 % FBS, 250 nM Dexamethazone, 0.5 mM Isobutylmethylxanthin, 10 ug/mL Insulin)
Taken after staining lipid droplets with Oil Red O
 GFP fluorescent cell imaging
Fluorescence and bright fieeld images (using a fluorescent imaging model of CytoWatcher) of NIH 3T3 cells (a 35 mm glass bottom dish) into which EGFP expression vector has been transfected.
 Strained tissue section
Masson trichrome stained section preparation of basal ganglia (mammal)